Characterization and phylogenetic analysis of antituberculous compound producing actinomycete strain D25 isolated from Thar Desert soil, Rajasthan
نویسندگان
چکیده
During the course of the anti-infective drug discovery programme, actinomycete strain D25 was recovered from the Thar Desert soil, Rajasthan, India. Actinomycin type of compound isolated from the strain D25 showed promising activity against multi drug resistant and extensively drug resistant M. tuberculosis isolates. The present study reports the characteristics and phylogenetic status of the actinomycete strain D25. Phenotypic and cell wall characteristics revealed that the strain belongs to the genus Streptomyces. Further 16s rRNA analysis confined the genus Streptomyces with 97% similarity to the closely related species Streptomyces althioticus KCTC 9752. The 16s rRNA sequence was submitted to GenBank with the accession number JN604533.1. According to Bossard et al. (2003) strain D25 was found to be a novel species of the genus Streptomyces from Thar Desert soil, Rajasthan.
منابع مشابه
Antibiotic Pigment from Desert Soil Actinomycetes; Biological Activity, Purification and Chemical Screening
An actinomycete strain, Streptomyces hygroscopicus subsp. ossamyceticus (strain D10) was isolated from Thar Desert soil, Rajasthan during the year 2006 and found to produce a yellow color pigment with antibiotic activity. Crude pigment was produced from strain D10 by solid state fermentation using wheat bran medium followed by extraction with ethyl acetate. The antimicrobial activity of the cru...
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Article history: Received on: 06/04/2015 Revised on: 22/04/2015 Accepted on: 17/05/2015 Available online: 27/06/2015 Effect of culture conditions and critical medium components on antitubercular pigment production from novel Streptomyces sp D25 isolated from Thar desert, Rajasthan was investigated. Antitubercular pigment from Streptomyces sp. D25 was produced by Agar Surface Fermentation (ASF) ...
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